Enzyme Linked antibody is attached to Antigen Anti body complex. The enzyme converts a substrate to a reaction product that emits photons of light instead of developing a visible color.
ECL is a unique and highly sensitive luminescence (light) detection system that amplifies the signal (you want) to detect ultra-low concentrations of analyte and reduces any signals you don’t want to deliver .
Electrochemiluminescence is the most sensitive detection method currently in use due to the ability of signal multiplication and amplification. Luminescent reactions are measured in relative light units (RLU) that are typically proportionate to the amount of analyte present in a sample.