HBA1C

HBA1C by HPLC

Now available at  Jagruthi…

Hexokinase & HBA1C at Jagruthi Rajahmundry

  • HbA1c is a form of hemoglobin that is measured primarily to identify the three month average plasma glucose concentration.

  • It is formed in a non-enzymatic glycation

Methods of testing HbA1c
Point of care ( POC) Instruments • Colorimetry ( Primitive methods )
Immunoassay methods ( CV 5-6 %)


HPLC – Gold standard in HbA1c Testing ( CV : 2-3 % )

Electrospray, iontophoresis and Mass spectrometry ( Not commercially available in India)

HPLC – High Performance Liquid Chromatography (HPLC)

at Jagruthi Rajahmundry

It is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material(stationary phase). 

HBA1C

  • Comprehensive Automated Hemoglobin testing

  • Combined Diabetes and Beta-Thalassemia

  • Simple efficient operation

  • Flexible and reliable operation

  • Whole blood primary tube sampling

 Advantages of HbA1c


• Index of long-term control over 120 days


• Can be done at any time of day


• Not influenced by diet, exercise, emotional disturbances on test day


• Useful index in clinical trials


• Useful if missed drugs / default diet


• Useful in stress hyperglycemia


• For HbA1c ,Fasting sample is not required.


• Low intra individual variability <2%.


• HbA1C sample is also stable; While blood sugar level is lowered unless precautions are taken.


• HbA1C value is not altered by Acute factors, while many factors will affect  blood sugar values.


• HbA1C is a better index for predicting complications. Because of all these reasons,HbA1C has become the preferred test nowadays.

 Biorad D10 – A fully automated HbA1c Testing by HPLC
• HbA1C quantitation in the presence of HbS, HbC and HbF.
• Minimum interference from carbamylation, lipemia and labile A1C.
• Traceable to the IFCC reference method.
• NGSP(National glycohaemoglobin standardisation programme) Certified

Reference values of HbA1c
Normal 4.5 -5.6%
prediabetics 5.7-6.4%
Diabetics – More than 6.5%
Adequate control -6.6-7%
Inadequate control -7-8%
Very poor control < 9% 

Summary:

For more details about various diagnostic facilities besides scanning facilites offered at our jagruthi Lab please click here to visit our homepage

To know more about HBA1C and related test details and its equipment at jagruthi Rajahmundry Biochemistry & Hexokinase Method.

 

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