Line Immuno Assay – for Allergy Diagnostics and Autoimmune Diagnostics

Allergy Testing

Now available at Jagruthi

Allergy Mechanism

Allergy Mechanism

  • IgE antibodies find allergen and attach themselves to it

  • Allergen bonded IgE binds to IgE receptors on surface of mast cells

  • Activated mast cells go through a process called degranulation. They release histamine and other chemicals causing Allergic reaction.

  • Newly formed mediators also released (prostaglandins, leukotrienes, thromboxanes and PAF

Allergy Testing

An allergy blood test is performed to determine if there is an allergic reaction to a known substance.Allergies occur when immune system over reacts to something in the environment. For example, pollen, which is normally harmless, can cause the body to overreact.

Common Symptoms of Allergy
  • a runny nose
  • sneezing
  • blocked sinuses
  • itchy, watery eyes
Types of Allergies
  • Urticaria
  • Dermatitis
  • Eczema
  • Hay fever
  • Conjunctivitis
  • Asthma (allergy-induced asthma)
Types of Allergens

Allergens are substances that can cause an allergic reaction.

  • Inhaled allergens.
  • Ingested allergens
  • Contact allergens.

Why allergy test is performed?

Allergies affect more than 50 million people living in the USA, according to the American College of Allergy, Asthma, and Immunology. Inhaled allergens are by far the most common type. Seasonal allergies and hay fever, which is an allergic response to pollen, affect more than 40 million Americans.

The World Allergy Organization estimates that asthma is responsible for 250,000 deaths annually. These deaths can be avoided with proper allergy care, as asthma is considered an allergic disease process.

Allergy testing can determine which particular pollens, molds, or other substances you’re allergic to. You may need medication to treat your allergies. Alternatively, you can try to avoid your allergy triggers.

Allergy in Children

Allergy Testing System Now Available at Jagruthi

Affordable and customised Allergy Panels to meet the Indian Scenario

Plexmat 4 – Immunoblot testing Equipment

PLEXMAT-4
PLEXMAT-4

  • Standardised incubation of immunoblot strips – higher precision and reproducibility

  • Automatisation immunoblot strips

  • validated autoantibody and allergology profiles

  • Easy operation

  • Combination of different conjugates/tests in one run

  • Walk-away function – fully automated from the start to the end of processing

  • Compatible with modern evaluation systems

EUROLineScan

Image analysis software for automated evaluation of membrane-based test systems

  • Fully automated identification, quantitation and assignment of bands.

  • For all areas: autoimmune diagnostics, infectious serology and allergy.

EUROLineScan

Two Allergy panels to meet most of the needs

Allergy Profile – Inhalation allergens, Class IgE

( India )

  1. Aspergillus fum.
  2. Carnation flower
  3. Cat
  4. Chicken feathers
  5. Cockroach, German
  6. Cotton (treated)
  7. Cultivated Corn
  8. Cultivated rye
  9. Der. farinae
  10. Dog
  11. Eucalyptus
  12. False ragweed
  13. House dust mite mix 1 (Der. ptero-nyssinus., Der. farinae)
  14. Jute
  15. Pigeon feathers
  16. Sheep’s wool
  17. Straw dust
  18. Sunflower
  19. Timothy grass
  20. Trichophyton m.
Allergy Profile – Food allergens –Class IgE
( India)
  1. Cow’s milk
  2. Egg yolk
  3. Milk powder
  4. Wheat flour
  5. Rice
  6. Soybean
  7. Peanut
  8. Coconut
  9. Apple
  10. Grape
  11. Potato
  12. Spinach
  13. Onion
  14. Cucumber
  15. Chicken
  16. Mustard
  17. Coffee
  18. Ginger
  19. Crab
  20. Shrimp / Prawn

ANA Profile Test For Differentiation of Antinuclear antibodies  (ANA)

Indications:

Sharp syndrome (Mixed connective tissue disease),

systemic lupus erythematosus,

Sjögren’s syndrome,

systemic sclerosis,

poly/dermatomyositis,

Primary biliary cholangitis (PBC).

Now Available at Jagruthi

Test strips  automatically incubated and evaluated using the systems Plexmat4, and EUROLineScan

ANA Blot
Antibodies against following Extractable Nuclear Antigens determined
  • nRNP/Sm
  • Sm
  • SS-A
  • Ro-52
  • SS-B
  • Scl-70
  • PM-Scl
  • Jo1
  • centromere protein B
  • PCNA
  • dsDNA
  • nucleosomes
  • histones
  • ribosomal P-proteins
  • AMA M2

Autoantibody Test

Results That Support an Autoimmune Disorder Diagnosis

Anti-RNP

Positive result seen in 95-100% of mixed connective tissue disease (MCTD) cases; may also be positive with lupus and scleroderma

Anti-Sm

Positive result seen in 30% of those with lupus; very specific antibody marker for this disease

Anti-SS-A (Ro)

Positive result seen in 75% of those with Sjögren syndrome; may also be positive with lupus and scleroderma

Anti-SS-B (La)

Positive result seen in 60% of those with Sjögren syndrome; may also be positive with lupus and scleroderma; rarely present without anti-SS-A

Anti-Scl-70

Positive result seen in 60% of those with scleroderma; highly specific antibody marker for this disease

Anti-Jo-1

Positive result seen in 30% of those with polymyositis; may be positive with pulmonary fibrosis

For more details about various diagnostic facilities besides scanning facilites offered at our jagruthi Lab please click here to visit our homepage

You can browse to know more about Allergy Testing and related test details and its equipment at jagruthi Rajahmundry Microbiology & Immunoassay

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